By Alexander Sulakvelidze, Elizabeth Kutter
Based on the emergence of pathogenic micro organism that can't be handled with present antibiotics, many researchers are revisiting using bacteriophages, or phages, to struggle multidrug-resistant micro organism. Bacteriophages: Biology and purposes presents exceptional, entire info on bacteriophages and their functions, akin to phage remedy. It bargains thoughts, media, and method focused on separating and dealing with healing phages. pictures, line drawings, and electron micrographs of phages also are incorporated. With its huge process, this ebook is an invaluable reference for microbiologists, hematologists, and infectious sickness researchers.
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Extra resources for Bacteriophages: Biology and Applications
Chemical mutagenesis in bacteriophage T2, Nature, 172, 964, 1953. Demerec, M. , Bacteriophage-resistant mutants in Escherichia coli, Genetics, 30, 119, 1945. , Sur un microbe invisible antagoniste des bacilles dysentériques. Compt Rend Acad Sci, 165, 373–375, 1917. , Le bactériophage: Son rôle dans limmunité, Masson et Cie, Paris, 1921. , Essai de traîtement de la peste bubonique par le bactériophage, La Presse Méd, 33, 1393–1394, 1925. , The Bacteriophage and its Behavior, Williams and Wilkins, Baltimore, MD, 1926.
Also, somewhat surprisingly, different assay hosts may give different estimates of the fraction of unneutralized phage. Adams suggests this may be due to minor differences in the receptors recognized on various hosts, leading to differences in bonding strength. Once determined for a given set of conditions, the K value can be used to calculate the appropriate dilution for any given experiment. With the aid of noted Russian electron microscopist Tamara Tikhonenko (Tikhonenko, Gachechiladze, Bespalova, Kretova, and Chanishvili, 1976), such techniques as immunoelectronmicroscopy were used to carry out particularly detailed studies of the process of inactivation by antibodies, as presented in Box 1.
6. 7. 1. 2. 3. 1. 2. 3. 4. 1. 2. 3. 4. 5. 1. 2. 1. 2. 3. 3. 1. 2. 3. 4. 8. 1. 1. THE NATURE OF BACTERIOPHAGES As discussed throughout this book, bacteriophages are viruses that only infect bacteria. They are like complex spaceships (Fig. 1), each carrying its genome from one susceptible bacterial cell to another in which it can direct the production of more phages. Each phage particle (virion) contains its nucleic acid genome (DNA or RNA) enclosed in a protein or lipoprotein coat, or capsid; the combined nucleic acid and capsid form the nucleocapsid.