Analyzing T Cell Responses: How to analyze cellular immune by Dirk Nagorsen, Francesco M. Marincola

By Dirk Nagorsen, Francesco M. Marincola

This publication experiences, describes, categorizes and gives a severe overview of all assays which have been used for the tracking of antigen-specific immune responses. particularly, it emphasizes a world method of the research of T phone mediated target/host interactions on the systemic and the peripheral point whilst such interactions are meant to take place. furthermore, the instruments defined the following may be applied for the hunt of surrogate biomarkers predictive of therapy responsiveness and/or scientific consequence which are of curiosity to the biotechnology undefined. intimately, this e-book presents an summary of antigen-specific immune biology in human types of tumor and viral ailment, discusses modulation of such responses via immune get away and offers mobile assays (cytoxicity, proliferation, cytokine construction utilizing ELISPOT, intracellular staining or cytometric evaluate, detection of antigen-specific T cells with tetrameric HLA/epitope complexes or MHC-IG dimers, T cellphone receptor research, evaluate of T telephone receptor/HLA interactions utilizing peptide/HLA-Green Fluorescent Protein complexes incorporation) and molecular assays together with quantitative real-time PCR and gene profiling for overview of systemic and peripheral immune responses. "This booklet represents the 1st complete description and assessment of crucial assays applied to watch immune responses opposed to tumor linked antigens. each one bankruptcy, ready by way of major investigators in tumor immunology and immunotherapy, information a selected immune subject or analytical approach. in particular adapted to the wishes of scientific researchers, uncomplicated scientist investigators, and biotechnology innovators, this ebook presents details that's either sufficiently distinct and eloquently succinct. All discussions are substantiated with a entire literature evaluation. The categorization and association of those sections is such that you'll both pursue the full publication or speedy locate precious info on a selected assay or suggestion of curiosity. a detailed description of the tools used in the id of tumor linked antigens is complemented by means of broad lists of HLA classification I, and sophistication II limited antigens. This booklet additionally addresses the fundamental subject of tumor cells?€™ skill to stay clear of immune reputation and destruction. the capability mechanisms concerning the disparity among the patients?€™ immunological and medical reaction following immunization are reviewed. Importantly, capability healing recommendations to beat the immune get away mechanisms were mentioned. In precis, examining T phone Responses offers the main gigantic and vast overview of immune response-monitoring concepts on hand to this point. This abundant details is successfully conveyed in a concise demeanour applicable for either starting investigators and skilled researchers."

Show description

Read or Download Analyzing T Cell Responses: How to analyze cellular immune responses against tumor associated antigens PDF

Best hematology books

Drawing Blood: Technology and Disease Identity in Twentieth-Century America

''Boldly and elegantly, Wailoo analyzes not just the position of physicians yet of analysis hospitals and pharmaceutical businesses. additionally, he indicates how such things as race, gender, and way of life motivated how physicians outlined and answered to the very illnesses that have been known as into lifestyles by way of the hot applied sciences they hired.

Meeting the Challenges of Blood Safety in the 21st Century: Pathogen Inactivation

Complement factor: Transfusion drugs and Hemotherapy 2004, Vol. 31, Suppl. 1

Molecular protocols in transfusion medicine

This crucial equipment handbook for immunohematologists (or hematologists and immunohematologists) offers info on genes that encode antigens on purple blood cells, platelets and neutrophils. The ebook starts off by means of overlaying common thoughts in molecular biology and particular protocols reminiscent of DNA guidance, PCR-RFLP and allele-specific PCR.

Cancer Cytogenetics, THIRD EDITION

The preeminent ebook on melanoma cytogenetics—now in a invaluable re-creation Like its winning predecessors, melanoma Cytogenetics, 3rd variation maintains to supply authoritative assurance of neoplastic tactics on the chromosomal point of genomic association. Now up-to-date and increased, this new version contains certain details at the latest advances within the box, incorporating an enormous quantity of latest cytogenetic in addition to molecular genetic information from the most recent easy and medical investigations.

Extra info for Analyzing T Cell Responses: How to analyze cellular immune responses against tumor associated antigens

Sample text

113. Oiso M, Eura M, Katsura F, et al. A newly identified MAGE-3-derived epitope recognized by HLA-A24- restricted cytotoxic T lymphocytes. Int J Cancer. 1999;81(3):387-394. 114. Bilsborough J, Panichelli C, Duffour MT, et al. A MAGE-3 peptide presented by HLAB44 is also recognized by cytolytic T lymphocytes m on HLA-B18. Tissue Antigens. Jul 2002;60(1):16-24. 115. Schultz ES, Zhang Y, Knowles R, et al. A MAGE-3 peptide recognized on HLA-B35 and HLA-A1 by cytolytic T lymphocytes. Tissue Antigens.

19. 20. 21. 22. 23. 24. 25. 26. 31 Chang CH, Flavell RA. Class II transactivator regulates the expression of multiple genes involved in antigen presentation. n J Exp Med. 1995;181(2):765-767. Chiari R, Hames G, Stroobant V, et al. Identification of a tumor-specific shared antigen derived from an Eph receptor and presented to CD4 T cells on HLA class II molecules. Cancer Res. 2000;60(17):4855-4863. Maccalli C, Li YF, El-Gamil M, Rosenberg SA, Robbins PF. Identification of a colorectal tumor-associated antigen (COA-1) recognized by CD4(+) T lymphocytes.

124. Gnjatic S, Nagata Y, Jager E, et al. Strategy for monitoring T cell responses to NYESO-1 in patients with any HLA class I allele. Proc Natl Acad Sci U S A. 2000;97(20):10917-10922. 125. Harada M, Li YF, El-Gamil M, Rosenberg SA, Robbins PF. Use of an in vitro immunoselected tumor line to identify shared melanoma antigens recognized by HLAA*0201-restricted T cells. Cancer Res. 2001;61(3):1089-1094. 126. Tsang KY, Zaremba S, Nieroda CA, Zhu MZ, Hamilton JM, Schlom J. Generation of human cytotoxic T cells specific for human carcinoembryonic antigen epitopes from patients immunized with recombinant vaccinia-CEA vaccine [see comments].

Download PDF sample

Rated 4.23 of 5 – based on 43 votes

About the Author

admin