100 Classic Houdini Tricks You Can Do by Joseph Dunninger

By Joseph Dunninger

A hundred vintage Houdini tips you are able to do (Fawcett ebook)

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In addition, some low-level resistance against CdC12 and potassium antimony1 tartrate was found in MRPS-transfected cell lines (McAleer et a1 1777). 001 et a1 LESSONS FROM ONCOLOGY 23 1999b). However, a preliminary report has suggested that MRP6 may mediate modest resistance to anthracyclines and epipodophyllotoxins (Belinsky et a1 2001). The MRP7, -8 and -9 transporter proteins were only very recently discovered and not much is yet known about their substrate preferences. BCRP is capable of transporting many anticancer agents.

This focuses interest on the problems in the remaining -30% of patients. - Changes in efflux transporter expression in epilepsy Immunocytochemical methods have shown presence of both Pgp (MDR1) and MRPl in epilepsy patients, in both brain endothelium and astrocytes (Tishler et a1 1995, Sisodiya et a1 1999, 2001), with evidence for up-regulation of Pgp. cDNA gene array analysis applied to brain endothelial cells from patients with medically intractable epilepsy showed overexpression of several multidrug resistance genes, with significant increases for M D R I , MRP2 and the cisplatin resistance-associated alpha protein, but no significant change in the case of M R P l or M D R 2 (Dombrowski et a1 2002).

Of the genuine MDR transporters, MDRl Pgp, BCRP and MRP1, only the first two are present at the apical membrane sections of polarized cells. MRPl , like the other MRP family members (except for MRP2), localizes laterally instead. As an example, in Fig. 2 normal human liver tissue is immunostained for both MRPG and MRP2. MRPG is present at the basolateral membranes of the hepatocytes (Fig. 2a), whereas MRP2 is present at the apical site of the hepatocyte, the canalicular membrane (Fig, 2b). An important implication of the opposite localization of MRPl is, that this transporter protects the surrounding tissue in a different way than MDRl Pgp and BCRP.

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